7LJ4

Human TRAAK K+ channel FHEIG mutant A270P in a K+ bound conductive conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	80
Detector technology	PIXEL
Collection date	2019-10-29
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97910
Spacegroup name	P 1 21 1
Unit cell lengths	80.280, 136.880, 95.580
Unit cell angles	90.00, 94.24, 90.00

Refinement procedure

Resolution	47.660 - 2.782
R-factor	0.21668
Rwork	0.214
R-free	0.25790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wfe
RMSD bond length	0.009
RMSD bond angle	1.528
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.660	47.659	3.087
High resolution limit [Å]	2.782	8.233	2.782
Rmerge	0.391	0.121	2.167
Rmeas	0.406	0.126	2.254
Rpim	0.112	0.035	0.617
Number of reflections	32708	2043	2044
<I/σ(I)>	4.69	18.505	1.323
Completeness [%]	63.2	99.41	15.03
Redundancy	13.2	6.46	6.63
CC(1/2)	0.992	0.983	0.554

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.8	277	50 mM Tris pH 8.8, 64-200 mM CaCl2, 27-33%(v/v) PEG400