7LIR
Structure of the invertebrate ALK GRD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-03-08 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9791 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 224.339, 224.339, 114.525 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.180 - 2.600 |
R-factor | 0.2255 |
Rwork | 0.223 |
R-free | 0.27010 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.594 |
Data reduction software | XDS (20200417) |
Data scaling software | SCALA (0.7.4) |
Phasing software | SHELX |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 112.420 | 50.180 | 2.700 |
High resolution limit [Å] | 2.600 | 9.720 | 2.600 |
Rmerge | 0.205 | 0.058 | 8.318 |
Rmeas | 0.209 | 0.060 | 8.479 |
Rpim | 0.040 | 0.012 | 1.632 |
Total number of observations | 22494 | 122935 | |
Number of reflections | 44990 | 959 | 4617 |
<I/σ(I)> | 14.9 | 58.5 | 0.5 |
Completeness [%] | 99.9 | 99.3 | 99.3 |
Redundancy | 26.9 | 23.5 | 26.6 |
CC(1/2) | 0.999 | 0.999 | 0.425 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 289.15 | 1.84 M (NH4)2SO4, Bicine pH 9.0, 28 mg/ml protein |