7LIP
X-ray structure of SPOP MATH domain (D140G)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-02-15 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.9794 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 84.766, 43.450, 51.026 |
Unit cell angles | 90.00, 123.22, 90.00 |
Refinement procedure
Resolution | 21.320 - 1.480 |
R-factor | 0.1823 |
Rwork | 0.180 |
R-free | 0.20520 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7lin |
RMSD bond length | 0.008 |
RMSD bond angle | 0.977 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (1.16_3549) |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 21.320 | 1.530 |
High resolution limit [Å] | 1.480 | 1.480 |
Rmerge | 0.085 | 0.660 |
Rmeas | 0.091 | 0.720 |
Rpim | 0.032 | 0.260 |
Number of reflections | 25043 | 2391 |
<I/σ(I)> | 20.56 | 3.24 |
Completeness [%] | 95.9 | |
Redundancy | 8.1 | |
CC(1/2) | 1.000 | 0.870 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | Reservoir solution: 0.1 M sodium citrate tribasic dihydrate, pH 5.6, 0.2 M (NH4)2SO4, 1 M Li2SO4. Two ul of the protein at 24 mg/ml in 20 mM Tris-HCl, pH 7.6, 150 mM NaCl, 5 mM DTT was mixed with 2 ul of the reservoir solution. The reservoir solution was 0.5 ml. |