7LI5

Crystal Structure Analysis of human TEAD1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	36.560, 89.320, 135.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.290 - 1.680
R-factor	0.1983
Rwork	0.197
R-free	0.22430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6im5
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	36.560	36.570	1.710
High resolution limit [Å]	1.680	4.560	1.680
Rmeas	0.074	0.034	1.739
Rpim	0.034	0.016	0.768
Total number of observations	223963	10569	11454
Number of reflections	50624	2602	2505
<I/σ(I)>	12.3	35.8	1.1
Completeness [%]	98.4	93.2	99.4
Redundancy	4.4	4.1	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	298	2.8 M Ammonium sulfate and 0.1 M Tris pH 9.0