7LHK

High-Resolution Crystal Structure of a Lipin/Pah Phosphatidic Acid Phosphatase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97934
Spacegroup name	C 2 2 21
Unit cell lengths	144.410, 171.626, 62.845
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.250 - 1.950
R-factor	0.1672
Rwork	0.165
R-free	0.21150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6tzy
RMSD bond length	0.011
RMSD bond angle	1.094
Data reduction software	DIALS
Data scaling software	Aimless (0.6.2)
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.250	2.020
High resolution limit [Å]	1.950	1.950
Rmerge	0.085	0.686
Rmeas	0.088	0.686
Rpim	0.024	0.238
Number of reflections	720604	5410
<I/σ(I)>	16.77	2.87
Completeness [%]	99.5	95.83
Redundancy	12.6	7.9
CC(1/2)	0.999	0.907

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	Tt Pah2 in a ratio of 2:1 with well solution of 0.1M sodium cacodylate pH 6.5, 1 M NaCl, 10% glycerol, 30% PEG 600