7LHK
High-Resolution Crystal Structure of a Lipin/Pah Phosphatidic Acid Phosphatase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-07-23 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97934 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 144.410, 171.626, 62.845 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.250 - 1.950 |
| R-factor | 0.1672 |
| Rwork | 0.165 |
| R-free | 0.21150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6tzy |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.094 |
| Data reduction software | DIALS |
| Data scaling software | Aimless (0.6.2) |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.250 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.085 | 0.686 |
| Rmeas | 0.088 | 0.686 |
| Rpim | 0.024 | 0.238 |
| Number of reflections | 720604 | 5410 |
| <I/σ(I)> | 16.77 | 2.87 |
| Completeness [%] | 99.5 | 95.83 |
| Redundancy | 12.6 | 7.9 |
| CC(1/2) | 0.999 | 0.907 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | Tt Pah2 in a ratio of 2:1 with well solution of 0.1M sodium cacodylate pH 6.5, 1 M NaCl, 10% glycerol, 30% PEG 600 |
