7LHE
Structure of full-length IP3R1 channel reconstituted into lipid nanodisc in the apo-state
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Energy Filter | GIF Bioquantum |
| Detector | GATAN K2 QUANTUM (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.3 |
| Software | cryoSPARC,RELION,PHENIX,EPU,CTFFIND,Coot |
| Symmetry | C4 |
| CTF correction | NONE |
