7LHA
Structure of the Exo-L-galactose-6-sulfatase BuS1_11 from Bacteroides uniformis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-05-14 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.9792 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 89.410, 89.410, 264.790 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.600 - 1.950 |
R-factor | 0.1505 |
Rwork | 0.148 |
R-free | 0.19350 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2qzu |
RMSD bond length | 0.009 |
RMSD bond angle | 0.949 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.000 | 1.990 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.166 | 0.920 |
Rpim | 0.053 | 0.178 |
Number of reflections | 79225 | 79363 |
<I/σ(I)> | 13.8 | 2.3 |
Completeness [%] | 100.0 | 100 |
Redundancy | 10.5 | 10.4 |
CC(1/2) | 0.993 | 0.920 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 0.1 M HEPES (pH 7.5), 0.2 M MgCl2, 25% (w/v) PEG 3350 |