7LH6
The structure of Bacteroides plebeius L-galactose dehydrogenase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-01-21 |
Detector | DECTRIS PILATUS 200K |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.754, 84.461, 129.499 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.870 - 2.850 |
R-factor | 0.2371 |
Rwork | 0.232 |
R-free | 0.28260 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ynp |
RMSD bond length | 0.003 |
RMSD bond angle | 0.714 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.900 |
High resolution limit [Å] | 2.850 | 2.850 |
Rmerge | 0.106 | 0.819 |
Rpim | 0.046 | 0.478 |
Number of reflections | 14732 | 704 |
<I/σ(I)> | 14.7 | 1.7 |
Completeness [%] | 98.6 | 95.5 |
Redundancy | 5.8 | 3.5 |
CC(1/2) | 0.994 | 0.820 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 0.2 M ammonium citrate dibasic (pH 5.5), 20% (w/v) PEG 3350 |