7LGN
Cyanophycin synthetase 1 from T. morbirosei
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-07-20 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9792 |
Spacegroup name | I 41 |
Unit cell lengths | 222.561, 222.561, 105.216 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 72.310 - 3.100 |
Rwork | 0.270 |
R-free | 0.32600 |
Structure solution method | SAD |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | CRANK2 |
Refinement software | REFMAC (5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 72.310 | 3.211 |
High resolution limit [Å] | 3.100 | 3.100 |
Rmerge | 0.067 | 0.404 |
Rmeas | 0.095 | 0.572 |
Rpim | 0.067 | 0.404 |
Number of reflections | 46806 | 4632 |
<I/σ(I)> | 11.47 | 0.8 |
Completeness [%] | 99.9 | 99.42 |
Redundancy | 2 | 2 |
CC(1/2) | 0.995 | 0.768 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.8 | 277 | 13.25% PEG3350, 320mM NaFormate, 1% glycerol, 100mM Na/K phosphate pH6.8. protein concentration 6mg/ml. |