7LGA
PEG10 CA-like C-terminal domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-04 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9791 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 51.598, 70.289, 52.689 |
Unit cell angles | 90.00, 98.10, 90.00 |
Refinement procedure
Resolution | 41.890 - 1.900 |
R-factor | 0.1842 |
Rwork | 0.182 |
R-free | 0.20930 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.763 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (1.18_3845) |
Refinement software | PHENIX (1.18_3845) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.930 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 58024 | 1166 |
<I/σ(I)> | 19.625 | |
Completeness [%] | 98.4 | |
Redundancy | 7.2 | |
CC(1/2) | 0.992 | 0.644 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M HEPES, pH 7.5 25% (w/v) PEG 3,350 0.2 M NaCl |