7LGA

PEG10 CA-like C-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9791
Spacegroup name	P 1 21 1
Unit cell lengths	51.598, 70.289, 52.689
Unit cell angles	90.00, 98.10, 90.00

Refinement procedure

Resolution	41.890 - 1.900
R-factor	0.1842
Rwork	0.182
R-free	0.20930
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.763
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.18_3845)
Refinement software	PHENIX (1.18_3845)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.930
High resolution limit [Å]	1.900	1.900
Number of reflections	58024	1166
<I/σ(I)>	19.625
Completeness [%]	98.4
Redundancy	7.2
CC(1/2)	0.992	0.644

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M HEPES, pH 7.5 25% (w/v) PEG 3,350 0.2 M NaCl