7LFZ

Human leukocyte antigen B*07:02 in complex with SARS-CoV2 epitope IPRRNVATL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2020-12-09
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9794
Spacegroup name	P 41 21 2
Unit cell lengths	65.636, 65.636, 237.472
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.820 - 1.900
R-factor	0.1819
Rwork	0.181
R-free	0.22390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5wmn
RMSD bond length	0.009
RMSD bond angle	1.510
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.840	1.940
High resolution limit [Å]	1.900	1.900
Rmerge	0.103
Rmeas	0.109
Rpim	0.035
Number of reflections	42071	2602
<I/σ(I)>	11.4	1.6
Completeness [%]	99.8	97.8
Redundancy	9.3	7.4
CC(1/2)	0.994	0.570

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	290	0.1 M sodium citrate, pH 8.0, 20% PEG4000, 20% isopropanol