7LFN
Structure of Hyperglycosylated Human IgG1 Fc (Fc267_329)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-1 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-12-15 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1.033190 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.483, 79.253, 141.321 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.750 - 2.600 |
R-factor | 0.2318 |
Rwork | 0.230 |
R-free | 0.26980 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5jii |
RMSD bond length | 0.010 |
RMSD bond angle | 1.052 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.750 | 2.720 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.044 | |
Rpim | 0.042 | 0.272 |
Number of reflections | 17601 | 2103 |
<I/σ(I)> | 11.8 | 2.6 |
Completeness [%] | 99.2 | |
Redundancy | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | CRYSTALS GROWN BY MIXING 1 UL OF FC267_329 (10 MG/ML IN 10mM HEPES, 75mM NaCl pH 7.4) WITH 1 UL OF PRECIPITANT SOLUTION CONSISTING OF 0.025M MES pH 7.0, 0.1M KCl, 20.571% w/v PEG3350 |