7LFI
MODEL OF MHC CLASS Ib H2-M3 WITH MOUSE ND1 N-TERMINAL HEPTAPEPTIDE REFINED AT 1.70 ANGSTROMS RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-01-04 |
| Detector | ADSC QUANTUM 1 |
| Wavelength(s) | 0.908 |
| Spacegroup name | P 1 |
| Unit cell lengths | 63.567, 68.646, 54.973 |
| Unit cell angles | 101.99, 95.10, 113.50 |
Refinement procedure
| Resolution | 33.530 - 1.700 |
| R-factor | 0.225 |
| Rwork | 0.223 |
| R-free | 0.25880 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1mhc |
| RMSD bond length | 0.013 |
| RMSD bond angle | 0.982 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.18.2-3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.530 | 1.720 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.119 | 0.571 |
| Number of reflections | 85212 | 1725 |
| <I/σ(I)> | 11.2 | 2.3 |
| Completeness [%] | 94.6 | 59 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 283 | 0.1 M Hepes, 20% (w/v) PEG 4000, 30% (v/v) ethylene glycol |
