7LFF
Crystal structure of the Candida albicans kinesin-8 motor domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-03-14 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 |
Unit cell lengths | 43.990, 52.680, 86.750 |
Unit cell angles | 86.90, 80.00, 89.92 |
Refinement procedure
Resolution | 45.910 - 2.010 |
R-factor | 0.2341 |
Rwork | 0.232 |
R-free | 0.26760 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3lre |
RMSD bond length | 0.005 |
RMSD bond angle | 1.047 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.910 | 2.080 |
High resolution limit [Å] | 2.010 | 2.010 |
Rmerge | 0.114 | |
Rmeas | 0.141 | |
Rpim | 0.082 | |
Number of reflections | 49650 | 19655 |
<I/σ(I)> | 4.6 | |
Completeness [%] | 97.4 | 96.7 |
Redundancy | 2.7 | |
CC(1/2) | 0.149 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 0.1 M MMT, 25% PEG 1500, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |