7LFD

Fab 7D6 bound to ApoL1 BH3 like peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	93
Detector technology	PIXEL
Collection date	2018-09-20
Detector	DECTRIS PILATUS 300K
Wavelength(s)	1.0
Spacegroup name	P 31 2 1
Unit cell lengths	129.947, 129.947, 90.817
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	70.674 - 2.157
R-factor	0.1676
Rwork	0.166
R-free	0.20280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7lfb
RMSD bond length	0.006
RMSD bond angle	0.958
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.674	2.273
High resolution limit [Å]	2.157	2.163
Rmerge	0.149	1.638
Rmeas	0.167	1.855
Rpim	0.074	0.855
Number of reflections	40614	2033
<I/σ(I)>	11.3	1.2
Completeness [%]	89.6
Redundancy	9.4
CC(1/2)	0.998	0.371

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	1.8M Ammonium Citrate pH 7.0