7LF8

Fab 6D12 bound to ApoL2 NTD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	93
Detector technology	PIXEL
Collection date	2018-04-25
Detector	DECTRIS PILATUS 300K
Wavelength(s)	0.98
Spacegroup name	C 2 2 21
Unit cell lengths	97.903, 154.290, 88.054
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.730 - 2.150
R-factor	0.194
Rwork	0.192
R-free	0.23000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7lf7
RMSD bond length	0.010
RMSD bond angle	1.100
Data reduction software	DIALS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	BUSTER (2.11.7)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	82.665	2.394
High resolution limit [Å]	2.150	2.160
Rmerge	0.286	1.776
Rmeas	0.325	2.102
Rpim	0.151	1.098
Number of reflections	25037	1254
<I/σ(I)>	17.7	1.4
Completeness [%]	93.5	69
Redundancy	8.5	6.3
CC(1/2)	0.989	0.106

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	20% PEG 3350, 0.2M Ammonium di-hydrogen phosphate, 14mM sodium cholate