Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-09-12 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.97952 |
| Spacegroup name | P 65 |
| Unit cell lengths | 196.690, 196.690, 476.330 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.170 - 5.990 |
| R-factor | 0.3838 |
| Rwork | 0.304 |
| R-free | 0.36810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ove |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.081 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2-3874_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.170 | 6.208 |
| High resolution limit [Å] | 5.990 | 5.995 |
| Number of reflections | 26042 | 2565 |
| <I/σ(I)> | 8 | |
| Completeness [%] | 99.5 | |
| Redundancy | 11.1 | |
| CC(1/2) | 0.997 | 0.777 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293.15 | 40% MPD, 100 mM Tris |
