7LDG
Crystal structure of the MEILB2-BRCA2 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-03-10 |
Detector | DECTRIS EIGER2 X 9M |
Wavelength(s) | 0.97895 |
Spacegroup name | H 3 2 |
Unit cell lengths | 179.889, 179.889, 179.186 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 31.100 - 2.560 |
Rwork | 0.211 |
R-free | 0.23070 |
Structure solution method | SAD |
RMSD bond length | 0.004 |
RMSD bond angle | 0.611 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1-3600) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 89.940 | 2.700 |
High resolution limit [Å] | 2.560 | 2.560 |
Rmerge | 0.088 | 1.467 |
Number of reflections | 35987 | 5188 |
<I/σ(I)> | 18.9 | 2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 21 | 21.7 |
CC(1/2) | 0.999 | 0.833 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 289 | 0.1 M sodium acetate (pH 4.6), 2 M lithium acetate |