7LCY
Crystal structure of the ligand-free ARM domain from Drosophila SARM1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-02-13 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9537 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 63.301, 101.363, 98.574 |
Unit cell angles | 90.00, 103.77, 90.00 |
Refinement procedure
Resolution | 47.870 - 3.350 |
R-factor | 0.2458 |
Rwork | 0.244 |
R-free | 0.27170 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.437 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.870 | 3.850 |
High resolution limit [Å] | 3.350 | 3.350 |
Number of reflections | 17198 | 3286 |
<I/σ(I)> | 4.7 | |
Completeness [%] | 97.7 | |
Redundancy | 3.4 | |
CC(1/2) | 0.996 | 0.373 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 1.7 M sodium malonate pH 5.8 |