7LCV

Factor H enhancing human antibody fragment (Fab) to meningococcal Factor H binding protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-23
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.11
Spacegroup name	P 1
Unit cell lengths	39.470, 52.370, 84.560
Unit cell angles	73.78, 84.48, 74.17

Refinement procedure

Resolution	81.180 - 1.700
R-factor	0.2401
Rwork	0.239
R-free	0.26970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3CVD and Fab homology model
RMSD bond length	0.017
RMSD bond angle	2.024
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	81.180	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.052	0.836
Rmeas	0.061	0.983
Rpim	0.032	0.513
Number of reflections	65915	6477
<I/σ(I)>	11.59	1.3
Completeness [%]	93.1	93.1
Redundancy	3.6	3.6
CC(1/2)	0.998	0.798

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	289	0.2 M LiNO3, 16% PEG 3350