7LCE

Structure of D-Glucosaminate-6-phosphate Ammonia-lyase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0000
Spacegroup name	P 2 2 21
Unit cell lengths	60.374, 171.569, 195.054
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.370 - 2.580
R-factor	0.1941
Rwork	0.193
R-free	0.22870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7lc0
RMSD bond length	0.002
RMSD bond angle	0.516
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.370	2.676
High resolution limit [Å]	2.580	2.580
Number of reflections	48940	8157
<I/σ(I)>	18.1
Completeness [%]	94.6
Redundancy	14.6
CC(1/2)	0.999	0.629

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	0.1 M Tris-HCl, pH 8.5, 0.4 M CaCl2, 11% PEG 4000, 8% glycerol