7LCB
C1B domain of Protein kinase C in complex with prostratin and phosphatidylcholine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 120 |
| Detector technology | PIXEL |
| Collection date | 2020-09-14 |
| Detector | Bruker PHOTON II |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 34.402, 25.869, 57.964 |
| Unit cell angles | 90.00, 97.29, 90.00 |
Refinement procedure
| Resolution | 28.750 - 1.700 |
| R-factor | 0.174 |
| Rwork | 0.172 |
| R-free | 0.21360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ptq |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.445 |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.600 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.056 | 0.264 |
| Number of reflections | 5115 | 441 |
| <I/σ(I)> | 15.48 | 3 |
| Completeness [%] | 89.1 | 48.4 |
| Redundancy | 3.85 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277.15 | Screen condition: 0.2 M Ammonium acetate 0.1 M Sodium Phosphate 30% Isopropanol pH 6.8; Drop condition: Protein: 2 mM in MES pH 6.5, 150 mM KCl; Phosphatidylcholine: 20 mM; Prostratin: 2.5 mM |
