7LC0

Structure of D-Glucosaminate-6-phosphate Ammonia-lyase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	54.089, 205.572, 164.026
Unit cell angles	90.00, 95.19, 90.00

Refinement procedure

Resolution	49.020 - 2.600
R-factor	0.1938
Rwork	0.193
R-free	0.22750
Structure solution method	SAD
RMSD bond length	0.001
RMSD bond angle	0.403
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.870	2.769
High resolution limit [Å]	2.600	2.600
Number of reflections	87468	4373
<I/σ(I)>	10.6
Completeness [%]	93.3
Redundancy	7.2
CC(1/2)	0.997	0.522

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.1 M MES, pH 6.5, 0.2 M Mg(OAc)2, 16% PEG 8000