7LA8

O6 variable lymphocyte receptor ectodomain bound to 3-HSO3-Gal-4GlcNAc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-16
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97915
Spacegroup name	I 4
Unit cell lengths	107.255, 107.255, 63.197
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.920 - 1.897
R-factor	0.186476030987
Rwork	0.184
R-free	0.22906
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rvx
RMSD bond length	0.015
RMSD bond angle	1.393
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.11_2567)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.200	2.010
High resolution limit [Å]	1.896	1.900
Rpim	0.048	0.855
Number of reflections	28496	4560
<I/σ(I)>	13.3	1.2
Completeness [%]	99.9
Redundancy	6.8
CC(1/2)	0.998	0.559

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	293.15	2.4 M (NH4)2SO4, 0.1 M Bicine pH 9.0, 20% glycerol