7LA7
O6 variable lymphocyte receptor ectodomain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-03-30 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.0332 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 52.450, 38.276, 78.690 |
Unit cell angles | 90.00, 103.79, 90.00 |
Refinement procedure
Resolution | 27.099 - 1.551 |
R-factor | 0.175958079504 |
Rwork | 0.174 |
R-free | 0.21484 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3g39 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.086 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.11_2567) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.100 | 1.610 |
High resolution limit [Å] | 1.550 | 1.550 |
Rpim | 0.044 | 0.501 |
Number of reflections | 125962 | 2093 |
<I/σ(I)> | 12.7 | 1.2 |
Completeness [%] | 99.6 | |
Redundancy | 5.7 | |
CC(1/2) | 0.894 | 0.533 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 10.5 | 293.15 | 1.2 M NaH2PO4, 0.8 M K2HPO4, 0.1 M glycine pH 10.5, 0.2 M Li2SO4 |