7L9C
Receiver Domain of RssB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-23 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.979110 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 46.459, 46.459, 95.847 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.100 - 1.850 |
| R-factor | 0.2031 |
| Rwork | 0.200 |
| R-free | 0.22620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6od1 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.110 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19_4080) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.230 | 1.890 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.050 | |
| Rmeas | 0.051 | |
| Rpim | 0.015 | |
| Number of reflections | 21241 | 977 |
| <I/σ(I)> | 24.77 | |
| Completeness [%] | 98.9 | 92.5 |
| Redundancy | 10.5 | |
| CC(1/2) | 0.999 | 0.800 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.8 | 298 | 0.2M lithium sulfate, 22% PEG 3,350, 0.1M Tris-HCl pH 8.8 |
