7L92
C1B domain of Protein kinase C in complex with diacylglycerol and dodecyl 2-(trimethylammonio)ethyl phosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 120 |
Detector technology | PIXEL |
Collection date | 2020-11-12 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.03322 |
Spacegroup name | H 3 |
Unit cell lengths | 89.068, 89.068, 218.679 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.960 - 1.750 |
R-factor | 0.216 |
Rwork | 0.214 |
R-free | 0.24600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ptq |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.600 | 1.780 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.070 | 0.613 |
Number of reflections | 64618 | 3574 |
<I/σ(I)> | 6.8 | |
Completeness [%] | 99.1 | |
Redundancy | 2.8 | |
CC(1/2) | 0.991 | 0.528 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277.15 | Screen condition: 0.2 M Ammonium acetate 0.1 M Sodium phosphate 15% Isopropanol pH 6.8 Drop conditions: For seed crystals Protein: 2 mM in MES pH 6.5, 150 mM KCl Dodecylphosphocholine: 20 mM 1,2-dioctanoyl-sn-glycerol: 2.5 mM Final drop Protein: 2 mM in MES pH 6.5, 150 mM KCl Dodecylphosphocholine: 10 mM 1,2-dioctanoyl-sn-glycerol: 2.5 mM |