7L8K
Crystal structure of human GPX4-U46C
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-11-18 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 32.777, 68.922, 35.725 |
Unit cell angles | 90.00, 116.12, 90.00 |
Refinement procedure
Resolution | 34.460 - 1.380 |
R-factor | 0.1487 |
Rwork | 0.147 |
R-free | 0.16510 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2obi |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.460 | 1.400 |
High resolution limit [Å] | 1.380 | 1.380 |
Number of reflections | 29060 | 1303 |
<I/σ(I)> | 18.9 | |
Completeness [%] | 99.9 | 93.9 |
Redundancy | 3.3 | 3 |
CC(1/2) | 0.990 | 0.940 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 8.2 | 293 | 0.056 M sodium phosphate monobasic monohydrate and 1.344 M potassium phosphate |