7L84
Hen Egg White Lysozyme by Native S-SAD at Room Temperature
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 295 |
Detector technology | PIXEL |
Collection date | 2019-07-17 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.892 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 79.344, 79.344, 37.810 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 56.100 - 1.700 |
R-factor | 0.1409 |
Rwork | 0.140 |
R-free | 0.16210 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 0.910 |
Data reduction software | DIALS (3.1.4) |
Data scaling software | Aimless (0.7.4) |
Phasing software | AutoSol (1.18.2_3874) |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 56.100 | 1.770 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.102 | 0.189 |
Rmeas | 0.103 | 0.208 |
Rpim | 0.015 | 0.084 |
Number of reflections | 22855 | 767 |
<I/σ(I)> | 35.63 | 6.7 |
Completeness [%] | 90.7 | 30.45 |
Redundancy | 35.7 | 5.9 |
CC(1/2) | 0.999 | 0.968 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 50 mM citrate buffer (pH 5.2 - 5.8), 20-25% PEG 4000, 20-25% glycerol |