7L7V
Crystal structure of Arabidopsis NRG1.1 CC-R domain K94E/K96E/R99E/K100E/R103E/K106E/K110E mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-04-04 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.03317 |
Spacegroup name | P 21 2 21 |
Unit cell lengths | 84.332, 89.656, 149.398 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.610 - 2.950 |
R-factor | 0.2205 |
Rwork | 0.219 |
R-free | 0.24030 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.628 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AutoSol |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.000 | 40.000 | 3.000 |
High resolution limit [Å] | 2.950 | 7.990 | 2.950 |
Rmerge | 0.130 | 0.045 | 1.687 |
Rmeas | 0.135 | 0.047 | 1.793 |
Rpim | 0.039 | 0.014 | 0.594 |
Total number of observations | 289192 | ||
Number of reflections | 24727 | 1371 | 1218 |
<I/σ(I)> | 4.9 | ||
Completeness [%] | 99.8 | 99.9 | 100 |
Redundancy | 11.7 | 11.4 | 8.5 |
CC(1/2) | 0.999 | 0.506 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M MES (pH 5.5), 1 M Potassium Sodium Tartrate |