7L79
Crystal structure of broadly HIV-1-neutralizing antibody VRC40.01
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-02-26 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.792, 135.950, 136.908 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.916 - 2.826 |
R-factor | 0.223 |
Rwork | 0.220 |
R-free | 0.27950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3se8 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (V1.1) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.850 |
High resolution limit [Å] | 2.800 | 7.590 | 2.800 |
Rmerge | 0.206 | 0.087 | 0.796 |
Rmeas | 0.241 | 0.103 | 0.948 |
Rpim | 0.124 | 0.055 | 0.508 |
Total number of observations | 104728 | ||
Number of reflections | 28072 | 1527 | 1338 |
<I/σ(I)> | 4.2 | ||
Completeness [%] | 98.5 | 97.3 | 98 |
Redundancy | 3.7 | 3.3 | 3.3 |
CC(1/2) | 0.991 | 0.668 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 10.6 % PEG 8000, 200 mM Zinc Acetate, Sodium Cacodylate, pH 6.5 |