7L78
H235C variant of Yeast Ferrochelatase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-03-20 |
Detector | DECTRIS PILATUS 300K |
Wavelength(s) | 0.98 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.987, 51.632, 287.336 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.926 - 2.400 |
R-factor | 0.236 |
Rwork | 0.233 |
R-free | 0.29660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1lbq |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.520 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 32665 | 3102 |
<I/σ(I)> | 17.7 | |
Completeness [%] | 99.2 | |
Redundancy | 3.2 | |
CC(1/2) | 0.995 | 0.795 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | PEG 2000, 2-propanol, Tris-HCl pH 7.5 |