7L6L
Crystal Structure of the DNA-binding Transcriptional Repressor DeoR from Escherichia coli str. K-12
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-10-17 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.751, 64.734, 140.710 |
| Unit cell angles | 90.00, 97.94, 90.00 |
Refinement procedure
| Resolution | 29.700 - 1.750 |
| R-factor | 0.1593 |
| Rwork | 0.158 |
| R-free | 0.18320 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.346 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.070 | 0.781 |
| Rmeas | 0.079 | 0.876 |
| Rpim | 0.036 | 0.393 |
| Number of reflections | 127632 | 6297 |
| <I/σ(I)> | 24 | 2.3 |
| Completeness [%] | 99.8 | 99.4 |
| Redundancy | 4.8 | 4.8 |
| CC(1/2) | 0.814 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 292 | Protein: 3.0 mg/ml, 0.5M Sodium chloride, 0.01M Tris pH 8.3, 5% Glycerol, TEV; Screen: Classics II (G5), 0.2M Lithium sulfate, 0.1M Tris pH 8.5, 25% (w/v) PEG 3350 |
