7L6J
Crystal Structure of the Putative Hydrolase from Stenotrophomonas maltophilia
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2020-06-19 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | I 41 3 2 |
Unit cell lengths | 171.693, 171.693, 171.693 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.450 - 1.780 |
R-factor | 0.1382 |
Rwork | 0.137 |
R-free | 0.15440 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.376 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.810 |
High resolution limit [Å] | 1.780 | 1.780 |
Rmerge | 0.111 | 0.886 |
Rmeas | 0.117 | 0.931 |
Rpim | 0.036 | 0.287 |
Number of reflections | 41390 | 2047 |
<I/σ(I)> | 23 | 3.3 |
Completeness [%] | 99.9 | 100 |
Redundancy | 10.3 | 10.4 |
CC(1/2) | 0.994 | 0.851 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 292 | Protein: 7.9 mg/ml, 0.5M Sodium chloride, 0.01M Tris pH 8.3; Screen: ComPAS (H12), 3.0M Sodium formate; Cryo: 4.0M Sodium formate. |