7L68
C-type carbohydrate-recognition domain 4 of the mannose receptor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-1 |
Synchrotron site | SSRL |
Beamline | BL12-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-09-12 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97946 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 31.623, 58.718, 62.383 |
Unit cell angles | 90.00, 97.66, 90.00 |
Refinement procedure
Resolution | 31.340 - 1.400 |
R-factor | 0.1558 |
Rwork | 0.155 |
R-free | 0.17490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7jub |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Refinement software | PHENIX (1.18.2) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 31.340 | 31.340 | 1.420 |
High resolution limit [Å] | 1.400 | 7.670 | 1.400 |
Rmerge | 0.057 | 0.082 | 0.111 |
Rmeas | 0.061 | 0.092 | 0.120 |
Rpim | 0.022 | 0.038 | 0.044 |
Total number of observations | 309250 | 1877 | 14754 |
Number of reflections | 42022 | 284 | 2009 |
<I/σ(I)> | 23.6 | 32.4 | 12.4 |
Completeness [%] | 94.4 | 97.9 | 92.6 |
Redundancy | 7.4 | 6.6 | 7.3 |
CC(1/2) | 0.997 | 0.972 | 0.990 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | Protein solution: 6mg/ml protein in 5mM CaCl2, 10mM Tris, pH 8.0, 25mM NaCl, and 10mM Man9GlcNAc2. Reservoir solution: 2.5% Peg 8K, 0.1M MES, pH 6.0 |