7L64

C-type carbohydrate-recognition domain 4 of the mannose receptor complexed with Lewis-a

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97946
Spacegroup name	P 1 21 1
Unit cell lengths	33.053, 57.180, 34.383
Unit cell angles	90.00, 112.81, 90.00

Refinement procedure

Resolution	30.470 - 1.350
R-factor	0.1662
Rwork	0.165
R-free	0.19840
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	7jub
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Refinement software	PHENIX (1.18.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.470	30.470	1.370
High resolution limit [Å]	1.350	7.390	1.350
Rmerge	0.042	0.068	0.147
Rmeas	0.045	0.079	0.159
Rpim	0.017	0.038	0.061
Total number of observations	178262	817	8843
Number of reflections	25718	155	1302
<I/σ(I)>	26.8	34	9.2
Completeness [%]	99.0	91.1	99
Redundancy	6.9	5.3	6.8
CC(1/2)	0.997	0.960	0.986

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	Protein solution: 6mg/ml protein in 5 mM CaCl2, 10 mM Tris, pH 8.0, 25 mM NaCl, and 20mM Lewis-a. Reservoir solution: 20% Peg 8K, 0.1M MES, pH 6.0