7L61

C-type carbohydrate-recognition domain 4 of the mannose receptor complexed with L-fucose-(alpha 1-2)-D-galactose-(beta1-4)-D-glucose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97946
Spacegroup name	P 1 21 1
Unit cell lengths	33.062, 56.525, 34.536
Unit cell angles	90.00, 112.94, 90.00

Refinement procedure

Resolution	30.450 - 1.350
R-factor	0.1618
Rwork	0.160
R-free	0.19040
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	7jub
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Refinement software	PHENIX (1.18.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.450	30.450	1.370
High resolution limit [Å]	1.350	7.390	1.350
Rmerge	0.049	0.082	0.087
Rmeas	0.054	0.096	0.094
Rpim	0.021	0.049	0.036
Total number of observations	176011	534	8671
Number of reflections	25483	125	1288
<I/σ(I)>	28.8	28.9	13
Completeness [%]	98.9	74.3	98.1
Redundancy	6.9	4.3	6.7
CC(1/2)	0.995	0.948	0.995

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	Protein solution: 6mg/ml protein in 5 mM CaCl2, 10mM Tris, pH 8.0, 25mM NaCl, and 15mM Fuc-alpha12-Gal-beta14-Glc. Reservoir solution: 12.5% Peg 8K, 0.1M MES, pH 6.0