7L4Y

YTH Domain of Human YTHDC1 with dsDNA Comprising Single N6mA joined by Two Six-bp DNA Duplexes in P212121 Crystal

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	45.968, 66.383, 77.605
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.550 - 1.790
R-factor	0.2087
Rwork	0.207
R-free	0.23970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6wea
RMSD bond length	0.003
RMSD bond angle	0.587
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.550	1.850
High resolution limit [Å]	1.790	1.790
Rmerge	0.094	0.794
Rpim	0.027	0.382
Number of reflections	20038	872
<I/σ(I)>	21.6	1.2
Completeness [%]	86.5	38.3
Redundancy	11.1	3.3
CC(1/2)		0.738

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	292	10% v/v 2-propanol, 26% PEG 400, and 0.1M sodium citrate tribasic dihydrate pH 5.0