7L4N
Crystal structure of the DRM2 (C397R)-CCG DNA complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-09-08 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9774 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 118.292, 231.692, 54.347 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.202 - 2.247 |
R-factor | 0.1817 |
Rwork | 0.180 |
R-free | 0.20950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4onj |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.330 |
High resolution limit [Å] | 2.247 | 4.850 | 2.250 |
Rmerge | 0.155 | 0.053 | 1.619 |
Rmeas | 0.166 | 0.057 | 1.754 |
Rpim | 0.060 | 0.020 | 0.643 |
Number of reflections | 35193 | 3581 | 3482 |
<I/σ(I)> | 3.2 | ||
Completeness [%] | 97.6 | 93.9 | 98.4 |
Redundancy | 7.2 | 7.3 | 6.8 |
CC(1/2) | 0.998 | 0.443 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 2% v/v Tacsimate(pH 6.0), 0.1 M BIS-TRIS (pH 6.5) and 20% w/v Polyethylene glycol 3,350. |