7L4M

Crystal structure of the DRM2-CCT DNA complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2018-06-15
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9774
Spacegroup name	C 1 2 1
Unit cell lengths	171.924, 70.981, 104.087
Unit cell angles	90.00, 97.90, 90.00

Refinement procedure

Resolution	47.056 - 2.805
R-factor	0.2168
Rwork	0.213
R-free	0.26300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4onj
RMSD bond length	0.004
RMSD bond angle	0.629
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.900
High resolution limit [Å]	2.800	6.030	2.800
Rmerge	0.098	0.049	0.998
Rmeas	0.114	0.057	1.174
Rpim	0.058	0.029	0.611
Total number of observations	108188
Number of reflections	30526	3172	2943
<I/σ(I)>	7.1
Completeness [%]	99.5	99.9	96.5
Redundancy	3.5	3.6	3.3
CC(1/2)		0.995	0.554

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277	0.1 M Sodium formate pH 7.0, 12% w/v Polyethylene glycol 3,350.