7L4L
Crosslinked Crystal Structure of Type II Fatty Acid Synthase Ketosynthase, FabF, and C8-crypto Acyl Carrier Protein, AcpP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-04-26 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 83.782, 92.136, 113.746 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.396 - 2.650 |
R-factor | 0.2243 |
Rwork | 0.222 |
R-free | 0.26650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6okg |
Data reduction software | MOSFLM |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.400 | 48.400 | 2.780 |
High resolution limit [Å] | 2.650 | 8.790 | 2.650 |
Rmerge | 0.404 | 0.084 | 1.899 |
Rmeas | 0.420 | 0.088 | 1.966 |
Rpim | 0.112 | 0.025 | 0.508 |
Total number of observations | 364390 | 9449 | 50899 |
Number of reflections | 26271 | 816 | 3428 |
<I/σ(I)> | 7.5 | 20.8 | 1.8 |
Completeness [%] | 100.0 | 99.6 | 100 |
Redundancy | 13.9 | 11.6 | 14.8 |
CC(1/2) | 0.988 | 0.998 | 0.728 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 281 | 30 % PEG 8K, 0.1 M sodium cacodylate pH 6.5, and 0.3 M sodium acetate |