7L4K
Crystal structure of the DRM2-CCG DNA complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-08-21 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9774 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 117.968, 231.582, 54.344 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.318 - 2.610 |
R-factor | 0.2156 |
Rwork | 0.213 |
R-free | 0.24030 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4onj |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.200 | 49.200 | 2.730 |
High resolution limit [Å] | 2.610 | 9.040 | 2.610 |
Rmerge | 0.190 | 0.035 | 1.639 |
Rmeas | 0.212 | 0.040 | 1.860 |
Rpim | 0.091 | 0.017 | 0.859 |
Total number of observations | 115890 | 2743 | 11827 |
Number of reflections | 23003 | 596 | 2753 |
<I/σ(I)> | 7.6 | 29.7 | 0.8 |
Completeness [%] | 99.5 | 95.8 | 99.3 |
Redundancy | 5 | 4.6 | 4.3 |
CC(1/2) | 0.990 | 0.998 | 0.409 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 2% v/v Tacsimate(pH 6.0), 0.1 M BIS-TRIS (pH 6.5) and 20% w/v Polyethylene glycol 3,350. |