7L4C
Crystal structure of the DRM2-CTT DNA complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-11-01 |
Detector | Nonius Kappa CCD |
Wavelength(s) | 0.9792 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 54.376, 232.512, 117.789 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.290 - 2.110 |
R-factor | 0.1867 |
Rwork | 0.185 |
R-free | 0.21490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4onj |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 48.290 | 2.170 |
High resolution limit [Å] | 2.110 | 8.950 | 2.110 |
Rmerge | 0.132 | 0.021 | 2.004 |
Rmeas | 0.145 | 0.024 | 2.221 |
Rpim | 0.059 | 0.010 | 0.939 |
Total number of observations | 3259 | 18265 | |
Number of reflections | 43203 | 646 | 3479 |
<I/σ(I)> | 10.7 | 46.8 | 0.9 |
Completeness [%] | 99.2 | 99 | 98.1 |
Redundancy | 5.8 | 5 | 5.3 |
CC(1/2) | 0.997 | 0.999 | 0.337 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 2% v/v Tacsimate TM (pH 6.0), 0.1 M BIS-TRIS (pH 6.5) and 20% w/v Polyethylene glycol 3,350. |