7L3O
Crystal Structure of the RNA binding domain of Threonyl-tRNA synthetase from Cryptosporidium parvum Iowa II
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2020-09-24 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.97872 |
Spacegroup name | P 1 |
Unit cell lengths | 65.730, 67.450, 143.180 |
Unit cell angles | 91.58, 97.53, 120.78 |
Refinement procedure
Resolution | 47.260 - 2.100 |
R-factor | 0.1788 |
Rwork | 0.178 |
R-free | 0.21290 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4hwt in two domains as per MoRDa |
RMSD bond length | 0.007 |
RMSD bond angle | 0.821 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MoRDa |
Refinement software | PHENIX (1.19rc4-4035) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.150 |
High resolution limit [Å] | 2.100 | 9.390 | 2.100 |
Rmerge | 0.062 | 0.037 | 0.422 |
Rmeas | 0.075 | 0.045 | 0.514 |
Number of reflections | 116442 | 1177 | 8551 |
<I/σ(I)> | 12.35 | 28.08 | 2.93 |
Completeness [%] | 95.8 | 87.8 | 95.2 |
Redundancy | 3.242 | 3.15 | 3.004 |
CC(1/2) | 0.997 | 0.995 | 0.851 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 287 | Microlytic MCSG1 screen e9: 200mM CaCl2, 20% (w/V) PEG 3350: CrpaA.18896.a.B3.PW38874 at 18mg/ml: tray 317798 e9: cryo: 20% EG in 2 steps: puck dxt2-9. |