7L3I
T4 Lysozyme L99A - propylbenzene - RT
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 278 |
Detector technology | PIXEL |
Collection date | 2018-03-15 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 60.977, 60.977, 97.268 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.774 - 1.460 |
R-factor | 0.1493 |
Rwork | 0.148 |
R-free | 0.16800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4w51 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.692 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER (2.8.2) |
Refinement software | PHENIX (1.14-3260) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.630 | 48.630 | 1.500 |
High resolution limit [Å] | 1.460 | 6.530 | 1.460 |
Rmerge | 0.065 | 0.040 | 0.865 |
Number of reflections | 37038 | 2028 | 1836 |
<I/σ(I)> | 13.5 | ||
Completeness [%] | 100.0 | 99.8 | 100 |
Redundancy | 6.6 | 6 | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | Crystals were grown from a 10 mg/mL frozen protein solution by the hanging drop method at 291-293K, with a 1:1 drop ratio of protein to solution and over a well solution of 0.1 M Tris-hydrochloride (pH 8), 20%-26% (w/v) PEG 4000, 70-170 mM lithium citrate, 8%-18% 2-propanol, 50 mM 2-mercaptoethanol, and 50 mM 2-hydroxyethyl disulfide |