7L1Z
Unlocking the structural features for the exo-xylobiosidase activity of an unusual GH11 member identified in a compost-derived consortium - NT-truncated form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-27 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.459 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 132.562, 149.686, 79.615 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.620 - 2.050 |
| R-factor | 0.1879 |
| Rwork | 0.186 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7l1w |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18rc2_3794) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.620 | 2.120 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmeas | 0.223 | 1.499 |
| Rpim | 0.061 | 0.427 |
| Number of reflections | 50051 | 4942 |
| <I/σ(I)> | 11.7 | |
| Completeness [%] | 100.0 | |
| Redundancy | 13.1 | |
| CC(1/2) | 0.997 | 0.641 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.1M sodium cacodylate trihydrate pH 6.5 and 30% (w/v) PEG 8000 |
