7L16
Crystal structure of sugar-bound melibiose permease MelB
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-05-19 |
Detector | DECTRIS PILATUS3 X 6M |
Wavelength(s) | 0.97741 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 127.372, 127.372, 103.905 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.350 - 3.150 |
R-factor | 0.2754 |
Rwork | 0.273 |
R-free | 0.29770 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | MelB D59C with aNPG submitted |
RMSD bond length | 0.002 |
RMSD bond angle | 0.520 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (dev_3936) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.350 | 3.370 |
High resolution limit [Å] | 3.150 | 3.150 |
Rmerge | 0.138 | 1.146 |
Rmeas | 0.146 | 1.539 |
Rpim | 0.046 | 0.477 |
Number of reflections | 16973 | 3080 |
<I/σ(I)> | 18.2 | 2.6 |
Completeness [%] | 99.9 | 100 |
Redundancy | 19.2 | |
CC(1/2) | 0.999 | 0.788 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 297.15 | 50 mM BaCl2, 50 mM CaCl2, 100 mM Tris-HCl, pH 8.5, 29-32% PEG 400, 0.015% DDMB, 10% PEG3350 |