7KZU
Quasi-intermediate state (Q) of a truncated Hsp70 DnaK fused with a substrate peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-12-14 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 145.135, 145.135, 122.212 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.510 - 2.150 |
R-factor | 0.2074 |
Rwork | 0.206 |
R-free | 0.24170 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4jne |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.32) |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.510 | 47.510 | 2.210 |
High resolution limit [Å] | 2.150 | 9.120 | 2.150 |
Rmerge | 0.178 | 0.033 | 5.004 |
Rmeas | 0.183 | 0.034 | 5.134 |
Rpim | 0.040 | 0.008 | 1.137 |
Total number of observations | 867680 | 11376 | 66268 |
Number of reflections | 41813 | 657 | 3341 |
<I/σ(I)> | 13.4 | 67.6 | 0.7 |
Completeness [%] | 100.0 | 99.4 | 99.5 |
Redundancy | 20.8 | 17.3 | 19.8 |
CC(1/2) | 0.999 | 1.000 | 0.349 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 0.1 M NaCl, 0.1 M HEPES pH 7.5, 1.6 M (NH4)2SO4 |