7KZH
Zinc finger antiviral protein (ZAP) central domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-28 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 89.637, 89.637, 53.055 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.810 - 2.490 |
| R-factor | 0.2279 |
| Rwork | 0.226 |
| R-free | 0.26310 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.529 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (1.18_3845) |
| Refinement software | PHENIX (1.18_3845) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.490 | 2.490 |
| Rmerge | 0.143 | 1.000 |
| Rmeas | 0.188 | 1.000 |
| Rpim | 0.028 | 0.244 |
| Number of reflections | 8779 | 435 |
| <I/σ(I)> | 24.2 | 5 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 48 | 24 |
| CC(1/2) | 0.998 | 0.893 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.8-1.1 M sodium nitrate 0.1 M sodium acetate |
