7KZE
Substrate-dependent divergence of leukotriene A4 hydrolase aminopeptidase activity
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-04-17 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 32 |
| Unit cell lengths | 139.888, 139.888, 84.343 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 53.840 - 2.900 |
| R-factor | 0.1922 |
| Rwork | 0.195 |
| R-free | 0.20880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ms6 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.635 |
| Data reduction software | PROTEUM |
| Data scaling software | PROTEUM |
| Phasing software | PROTEUM |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.840 | 3.004 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.140 | 0.320 |
| Number of reflections | 80258 | 40210 |
| <I/σ(I)> | 11.41 | |
| Completeness [%] | 98.0 | |
| Redundancy | 9.75 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295.15 | 60-75 mM magnesium formate dihydrate, 19-23 % PEG3350 |
